General description

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC₅₀ = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC₅₀ = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Canu, N., et al. 2005. J. Neurochem.92, 1228.Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.Beugnet, A., et al. 2003. Biochem. J.372, 555.Eskelinen, E.L., et al. 2002. Traffic3, 878.Petiot, A., et al. 2000. J. Biol. Chem.275, 992.Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.

Packaging

100 mg in Glass bottle

Packaged under inert gas

50 mg in Plastic ampoule

Preparation Note

In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)